Chao Liang, Yuanchang Liu, Chong Liu, Xia Li, Li Chen, Chunzheng Duan, Jingmin Li.Analysis of Magnetic Microbead Capture With and Without Bacteria in a Microfluidic Device Under Different Flow Scenarios. Combining dielectrophoresis and concentration polarization-based preconcentration to enhance bead-based immunoassay sensitivity. Electroosmotic flow driven microfluidic device for bacteria isolation using magnetic microbeads. Analytical and Bioanalytical Chemistry 2021, 80 A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac. Alexander Ecke, Tanja Westphalen, Jane Hornung, Michael Voetz, Rudolf J.Improved Specific Biodetection with Ion Trap Mobility Spectrometry (ITMS): A 10-min, Multiplexed, Immunomagnetic ELISA.
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Limits of detection for ovalbumin and botulinum toxoid individually were 4 (or 80 pg) and 16 ng/mL (or 320 pg), respectively, using the manual assay. Released eTags are analyzed using capillary gel electrophoresis with laser-induced fluorescence detection.
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Upon excitation at 680 nm, free radicals are generated, which diffuse and cleave the linkage, releasing the eTags. In the presence of antigen, a molecular complex is formed where the cleavable linkage is held in proximity to the photoactive group. Aqueous samples are incubated with the mixture of antibodies along with streptavidin-coated magnetic beads and a photoactive porphyrin complex. Detection is based on two antibodies binding simultaneously to a single antigen, one of which is labeled with biotin while the other is conjugated to a fluorescent eTag through a cleavable linkage. To detect toxins, we modified and automated an antibody-based approach previously developed for manual medical diagnostics that uses fluorescent eTag reporter molecules and is suitable for highly multiplexed assays. We are developing an automated system for the simultaneous, rapid detection of a group of select agents and toxins in the environment.